Coding
CotB

Part:BBa_K4733008:Design

Designed by: Diego Perez Hidalgo   Group: iGEM23_UBC-Okanagan   (2023-10-05)


Spore Coat Protein B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 911
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 198


Design Notes

The UBC-Okanagan 2023 team used Modular Cloning (MoClo) standardization for this part: added Type II restriction sites and standard flanks to this part.


Source

The sequence for CotB is available in the UniProt database. It was sourced from Bacillus subtilis (strain 168) by Presecan et al. (1997).

References

Han, M., & Enomoto, K. (2010). Surface display of recombinant protein on the cell surface of Bacillus subtilis by the CotB anchor protein. World Journal of Microbiology and Biotechnology, 27(3), 719–726. https://doi.org/10.1007/s11274-010-0490-8

Guoyan, Zhang, et al. “Bacillus Subtilis Spore Surface Display Technology: A Review of Its Development and Applications.” Journal of Microbiology and Biotechnology, vol. 29, no. 2, 28 Feb. 2019, pp. 179–190, https://doi.org/10.4014/jmb.1807.06066. Accessed 3 Nov. 2020.

Presecan, E., Moszer, I., Boursier, L., Ramos, H. C., de la Fuente, V., Hullo, M.-F. ., Lelong, C., Schleich, S., Sekowska, A., Song, B. H., Villani, G., Kunst, F., Danchin, A., & Glaser, P. (1997). The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees). Microbiology (Reading, England), 143 ( Pt 10), 3313–3328. https://doi.org/10.1099/00221287-143-10-3313

Ricca, Ezio, and Simon M Cutting. Journal of Nanobiotechnology, vol. 1, no. 1, 2003, p. 6, https://doi.org/10.1186/1477-3155-1-6. Accessed 3 Nov. 2020.

Hinc, Krzysztof, et al. “Efficient Binding of Nickel Ions to Recombinant Bacillus Subtilis Spores.” Research in Microbiology, vol. 161, no. 9, Nov. 2010, pp. 757–764, https://doi.org/10.1016/j.resmic.2010.07.008. Accessed 10 Apr. 2023.

Su, Yuan, et al. “Bacillus Subtilis: A Universal Cell Factory for Industry, Agriculture, Biomaterials and Medicine.” Microbial Cell Factories, vol. 19, no. 1, 3 Sept. 2020, microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-020-01436-8, https://doi.org/10.1186/s12934-020-01436-8.

The University of Michigan. “OPM.” Opm.phar.umich.edu, 2005, opm.phar.umich.edu/ppm_server3_cgopm. Accessed 12 Oct. 2023.